.  ÉTUDES

https://www.youtube.com/watch?v=98SCiVYX5yg&feature=youtu.be

 Tous nos implants  sont répertoriés sur le site web OsseoSource.

Dispositifs médicaux classe II B-CE 0426. Directive 93/42/CEEUNI EN ISO 13485-2004 (ISO 13485-2003).

Nos implants dentaires ne sont pas des produits low cost.

Attention certains fabricants utilisent le terme GENERIQUE ,mais ce sont des produits low cost.

      

TRAITEMENT DE SURFACE :

University of Trieste carried out several tests ondental implants manufactured by GiesseTechnology.
The surface of Giesse implantsis processed by performing the HSS(High Speed​​Surface)treatment.
It consists of afirst step of sand blasting and sub sequently in an acid treatment.
HSS allows to havea greater wettability of the implant surface and ahomogeneous roughness produced by sand blasting and consequently a better bone regeneration,compared to a system with only sand blasted surfaceas we can seein the pictures below.

    

Wettability comparison bet ween implantonly san ded (left) and HSS treated (right). The lower contact angle be tween drop of serum forcell cultures and surface corresponds to better wettability.

     

Implant surface comparison: sand blasted only (left) and HSS  treated (5000X magnification).

The homogeneity of the asperities and their better distribution on the HSS surface compared to only sandblasted.

The observations made by SEM are confirmed by the quantitative data supplied by surface profile.

 

 

Sandblasted samples

HSS samples

Ra  (μm)

1.043±0.123

2.151±0.202

Rsk

‐0.143±0.589

‐0.038±0.168

Rku

4.488±1.656

2.887±0.258

 The average roughness, Ra, shows a value statistically lower than the sandblasted surface treatment compared to the HSS. The roughness generated by the sand are lower than those generated by the HSS treatment.

The standard deviations of the two roughness Kurtosis are high different for the two surface treatments. This is a further indication of the greater uniformity of the HSS treatment.

The samples treated HSS have a better purity compared to only sandblasted surface and a marked presence of phosphorous due to acid action performed on the surface. According to the literature this action could promote cell adhesion by the osteoblasts and increase quality osseointegration.

As regards the evaluation of the fixture-abutment fitting, tests allow to conclude the connection abutment-implant is highly coupled, with few and very reduced spaces between abutment and implant collar. These spaces are not sufficient to ensure penetration of bacterial agents. For these tests were considered implants with conical connection, technologically more complex to manufacture.

                     

                            

Finally it was performed a test of cellular proliferation where it’s clearly visible the high level of adhesion to the substrate by the osteoblasts and the presence of many filopodia (in greater amounts as compared to implants only sandblasted). This allows a fast and excellent osseointegration of HSS implant (in red in the graph below) especially in the first days after surgery, thus increasing the chances of success, compared to a standard implant only sandblasted (in blue), both compared to a control (green).

                       

Conditions de réalisation des actes d’implantologie orale

 N. 001 VALIDATION METHODE DE STERILISATION

N. 141 TESTS COMPARATIFS TECOM NOBEL BIOCARE SURFACES

N.408 ETUDE COMPARATIF SURFACES VERSUS STRAUMANN

    

MECHANICAL TESTS

 N. 337 ADHESION CELLS SANDBLASTED IMPLANTS TEST

N. 357 CYTOTOXICITY TEST

  

 N. 362 ADHESION CELLS TU IMPLANTS TEST

N. 435 BIOBURDEN TEST

N. 437 REALTIME PCR TU IMPLANTS TEST

N. 440 XPS TEST

N. 538 REALTIME PCR SANDBLASTED IMPLANTS TEST

N. 852 PYROGEN FREE TEST

N. 12527 BIOBURDEN TEST

N. 13391 STERILITY TEST

PRODUCTION PLAN

In vitro studies      

Our cell culture lab offers cytotoxicity testing, evaluation of cell adhesion and growth, evaluation of cellular activity on implant materials surfaces, using the most suitable cell lines. Characterization involves optical and phase contrast microscopy, SEM and fluorescence microscopy. Cell activity and metabolism are evaluated through specific microplate tests or by gene expression through RT-PCR and PCR arrays

Adherent endotoxin on dental implant surfaces: a reappraisal.
Morra M, Cassinelli C, Cascardo G, Bollati D, Bellanda M.
J Oral Implantol. 2012 Nov 12. [Epub ahead of print].

Effects of type I collagen coating on titanium osseointegration: histomorphometric, cellular and molecular analyses.
Sverzut AT, Crippa GE, Morra M, de Oliveira PT, Beloti MM, Rosa AL.
Biomed Mater. 2012 Jun;7(3)

Affecting osteoblastic responses with in vivo engineered potato pectin fragments.
Kokkonen H, Verhoef R, Kauppinen K, Muhonen V, Jørgensen B, Damager I, Schols HA, Morra M, Ulvskov P, Tuukkanen J..
J Biomed Mater Res A. 2012 Jan;100(1):111-9

Gene expression of markers of osteogenic differentiation of human mesenchymal cells on collagen I-modified microrough titanium surfaces.
Morra M, Cassinelli C, Cascardo G, Bollati D, Baena RR.
J Biomed Mater Res A. 2011 Feb;96(2):449-55.

Multifunctional implant surfaces: surface characterization and bone response to acid-etched Ti implants surface-modified by fibrillar collagen I.
Morra M, Cassinelli C, Cascardo G, Bollati D, Rodriguez Y Baena R.
J Biomed Mater Res A. 2010 Jul;94(1):271-9.

Pectin-coated titanium implants are well-tolerated in vivo.
Kokkonen H, Niiranen H, Schols HA, Morra M, Stenbäck F, Tuukkanen J.
J Biomed Mater Res A. 2010 Jun 15;93(4):1404-9

Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored pectins.
Gallet M, Vayssade M, Morra M, Verhoef R, Perrone S, Cascardo G, Vigneron P, Schols HA, Nagel MD.
Acta Biomater. 2009 Sep;5(7):2618-22.

Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface.
de Assis AF, Beloti MM, Crippa GE, de Oliveira PT, Morra M, Rosa AL.
Clin Oral Implants Res. 2009 Mar;20(3):240-6.

Covalently-linked hyaluronan promotes bone formation around Ti implants in a rabbit model.
Morra M, Cassinelli C, Cascardo G, Fini M, Giavaresi G, Giardino R.
J Orthop Res. 2009 May;27(5):657-63

Differentiation of osteoblasts on pectin-coated titanium.
Kokkonen H, Cassinelli C, Verhoef R, Morra M, Schols HA, Tuukkanen J.
Biomacromolecules. 2008 Sep;9(9):2369-76.